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Structured Review

5 PRIME phase lock gel-heavy tubes
Phase Lock Gel Heavy Tubes, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phase+lock+gel-heavy+tubes/phase+lock+gel+tubes/pm40659225-104-11-12
Average 90 stars, based on 1 article reviews
phase lock gel-heavy tubes - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Purification:

Article Title: Selective sequestration of signaling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus
Article Snippet: .. The pellets were resuspended in TRIzol (Ambion) and extracted using Phase Lock Gel-Heavy tubes (5 PRIME), and RNA was purified using the RNA Clean and Concentrator-5 kit (Zymo Research). ..

Article Title: A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues
Article Snippet: .. The pellets were then resuspended in TRIzol (Ambion) and extracted using Phase Lock Gel-Heavy tubes (5 PRIME), and RNA was purified using the RNA Clean and Concentrator-5 kit (Zymo Research). .. Genomic DNA was eliminated by treating the samples twice with 1 MBU Baseline-ZERO DNAse (Epicentre) at 37 °C for ∼20 min. RNA was recollected using Zymo-Spin columns and subsequently reverse transcribed using the SuperScript III reverse transcriptase kit (Invitrogen).

Incubation:

Article Title: Drug-induced chromatin accessibility changes associate with sensitivity to liver tumor promotion
Article Snippet: Samples were homogenized using a MagNA Lyser homogenizer (Roche) using two runs of 30 s at 6,500 rpm. .. Aliquots of ∼600 μl of homogenized material were transferred into pre-spun 2-ml Phase Lock Gel-Heavy tubes (5Prime) and incubated for 5 min at room temperature. ..

other:

Article Title: Mechanical regulation of cardiac fibroblast profibrotic phenotypes
Article Snippet: The cell lysate was then added to prespun Phase Lock Gel-Heavy tubes (cat. no. 2302830; 5 Prime GmbH, Hamburg, Germany), spun at 1500 × g for 30 s, and incubated for 5 min at 22°C.

Article Title: Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus.
Article Snippet: The pellets were resuspended in TRIzol (Ambion) and extracted using Phase Lock Gel-Heavy Tubes (5Prime), and RNA was purified using the RNA Clean and Concentrator-5 kit (Zymo Research).

Article Title: Vascular Morphogenesis
Article Snippet: Phase Lock Gel-Heavy 1.5 ml tubes (5PRIME).

Isolation:

Article Title: Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)
Article Snippet: .. RNA was isolated using a phenol-based extraction method (TRIzol reagent; Invitrogen, Burlington, ON, Canada) and Phase Lock Gel-Heavy tubes for phase separation of RNA, per the manufacturer’s instructions (5 Prime Inc., Gaithersburg, MD). .. Total RNA was purified using an RNeasy minikit (Qiagen, Germantown, MD) and eluted in 50 l of RNase-free water, per the manufacturer’s instructions.

Extraction:

Article Title: Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)
Article Snippet: .. RNA was isolated using a phenol-based extraction method (TRIzol reagent; Invitrogen, Burlington, ON, Canada) and Phase Lock Gel-Heavy tubes for phase separation of RNA, per the manufacturer’s instructions (5 Prime Inc., Gaithersburg, MD). .. Total RNA was purified using an RNeasy minikit (Qiagen, Germantown, MD) and eluted in 50 l of RNase-free water, per the manufacturer’s instructions.



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